To facilitate homeostasis for gametes and embryos the GIII Series™ has been developed. The media of the GIII Series™ all have the same ionic backbone resulting in similar osmolality from oocyte aspiration and sperm preparation to embryo culture and transfer.

All IVF media of the GIII Series™, with the exception of G-RINSE™ and EmbryoGlue®, require albumin supplementation either with G-MM™ or HSA-solution™.

As a blood product, HSA is a risk factor in embryo culture. Even though albumin is tested for HIV, hepatitis A, B and C, there is no reliable test for prion transmitted disease such as Creutzfeld Jacobs disease. To circumvent the risks of HSA, recombinant human albumin (r-HA) has been recently introduced for media supplementation.

G-MM™ contains recombinant human albumin in the concentration of
50 mg/mL. HSA-solution™ is human serum albumin in the concentration of 100 mg/mL.

Oocyte retrieval and handling media
Before oocyte retrieval, the cervix and utensils should be washed using G-RINSE™.

For oocyte retrieval and for handling of gametes and embryos G-MOPS™ has been designed. G-MOPS™ contains the carbohydrates and amino acids that are required to support the gametes and embryos and contains a buffer to maintain pH in an air atmosphere. After oocyte identification, the oocytes should be rinsed in G-MOPS™ and transferred to G-FERT™.

Sperm preparation media

For sperm preparation, G-SPERM™ should be used. G-SPERM™ is designed to support the washing and purification of sperm. G-SPERM™ contains Gentamicin, the carbohydrates required to support sperm function and Hyaluronan. After washing, the sperm should be diluted in G-FERT™ for fertilization.

Fertilization Media

G-FERT™ is designed to support the preparation of gametes and in vitro fertilization. G-FERT™ contains the carbohydrates and amino acids to support both male and female gametes. G-FERT™ also contains Hyaluronan.

Optimized culture conditions

After fertilization, continued culture takes place in G-1™ version 3 until transfer on day 2 or 3. G-1™ version 3 is designed to support the development of cleavage stage embryos to around the 8-cell stage. G-1™ version 3 contains Hyaluronan, carbohydrates, amino acids and chelators to support the early embryo.

For continued culture to the blastocyst stage G-2™ version 3 should be used. G-2™ version 3 is designed to support the development of the embryo from around the 8-cell stage to the blastocyst stage. G-2™ version 3 contains Hyaluronan, carbohydrates, amino acids and vitamins to support the later stage embryo.

For optimal embryo culture, droplets under oil are preferred. For covering of droplets G-OIL™ is recommended. G-OIL™ is liquid paraffin oil designed for use as an overlay for media.

New embryo transfer medium

For embryo transfer EmbryoGlue® has been designed. EmbryoGlue® contains carbohydrates, amino acids, vitamins and Hyaluronan.

G-RINSE™ IVF Media

Solution for rinsing of contact materials and for washing of the cervix. Not for culture.

Characteristics
No protein supplementation needed.

G-MOPS™

Medium for handling and manipulating oocytes and embryos in ambient atmosphere.

Characteristics
Contains NEAA and G, P, L and MOPS.

No pre-equilibration in CO2 needed. No protein supplementation needed for oocyte retrieval. For embryo manipulation after the addition of G-MM™ or HSA-solution™.

G-SPERM™

Medium for sperm preparation.

Characteristics
Contains HA, NEAA, G, P, L and Gentamicin